Introduction to Biotechnology
In labs so bright with glass so clean,
A science blooms—alive, unseen.
With genes as letters, life’s own code,
We shape the path where futures load.
Biotechnology, a marvel so vast,
Merging old ways with knowledge amassed.
From microbes small to plants so tall,
A world of wonders, one and all.
We tweak the genes, we mend, we cure,
For food and health, and much, much more!
Principles of Biotechnology
With two key tools, this field does shine,
Gene techniques and cell design.
One takes the genes from here and there,
While cultures grow with utmost care.
Genetic Engineering, a magic spell,
Where genes are changed to serve us well.
Recombinant DNA, a crafty art,
Takes one gene and makes it part.
Bioprocess Engineering, in tanks so wide,
Helps cells grow and thrive inside.
From enzymes pure to hormones fine,
These bio-factories work in line.
Tools of Genetic Engineering
Like artists hold their brush and pen,
Scientists need their tools again.
Restriction enzymes, precise and keen,
Cut DNA where lines are seen.
Vectors help these fragments move,
Like tiny taxis, smooth and groove.
Plasmids carry genes so light,
While bacteriophages join the fight.
DNA Ligase, the glue so strong,
Binds new genes, where they belong.
With PCR, the copies rise,
Multiplying before our eyes!
Host organisms, they must be fit,
To take new genes and live with it.
Bacteria and yeast, so quick to grow,
Form the base for what we sow.
Steps in Genetic Engineering
The journey starts, we take a stand,
To modify life with careful hand.
Isolation of DNA, pure and bright,
Extracted with care, a precious sight.Cutting the gene, a measured slice,
With enzymes acting cold as ice.Inserting it into a vector’s frame,
Where new blueprints stake their claim.Introducing it inside a cell,
Hoping all goes safe and well.Selection and growth, so neat, so fine,
Identifying success in a genetic line.
Recombinant DNA Technology (rDNA Technology)
DNA recombines, a tale untold,
From past to future, new and bold.
With plasmid rings and clever tricks,
We gift new traits like magic mix.
Bacteria play their helpful part,
Multiplying genes with all their heart.
Insulin, vaccines, crops so grand,
Rise from these cells, well-planned.
Bioreactors – The Factories of Life
Behold the tanks where wonders grow,
With air and food in measured flow.
Like bubbling pots, they swirl and spin,
Producing proteins locked within.
Stirred tanks mix and blend,
While spargers help with air to send.
Microbes feast and multiply,
Yielding goods in bulk supply.
Downstream Processing – The Final Touch
A product fine, yet not complete,
Needs polishing before it’s neat.
Filtration first, to clear the way,
Then purification to have its say.
Drying, packaging, tested right,
Ensuring standards are met so tight.
Ready it goes, to serve and heal,
A wonder born, a grand appeal!
Conclusion
So here we stand, with knowledge bright,
A science woven from genes in light.
Biotechnology, a boon so vast,
Shaping futures, unsurpassed!
With care we craft, with mind we mold,
A world rewritten, bold and gold.
Where healing, farming, all entwine,
In strands of life, by grand design!
Also Read: Microbes in Human Welfare - A Poetic Tale
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